Turkenkopf I J, Johnson P R, Greenwood M R
Am J Physiol. 1982 Apr;242(4):E220-5. doi: 10.1152/ajpendo.1982.242.4.E220.
Pancreatic and plasma insulin concentrations in preobese and lean Zucker rats were determined during three developmental periods: 1) gestation, 2) suckling, and 3) postweaning. Individual fetal samples were derived from two types of matings: 1) homozygous obese males (fafa) with heterozygous females (Fafa), and 2) homozygous lean males and females (FaFa). Suckling and postweaned pups from similar matings were partially pancreatectomized, tail bled, and identified retrospectively. In 21-day-old fetuses bearing the fa gene, plasma insulin concentration was elevated (P less than 0.001) and pancreatic concentration was slightly lower (P less than 0.05) compared to homozygous lean fetuses. Neither pancreatic nor plasma insulin concentration differed between preobese and lean pups during suckling, except that pancreatic concentration became elevated in preobese pups on postnatal day 20 (P less than 0.05). Plasma insulin concentration was elevated 24 h postweaning (P less than 0.01). These data support the hypothesis that the fa gene initiates metabolic changes during gestation that are modulated during suckling, but reappear at weaning. The data also establish that increased adipocyte size and lipoprotein lipase activity in 7- to 12-day-old preobese pups are not dependent on concomitant hyperinsulinemia.
在三个发育阶段测定了肥胖前期和瘦型 Zucker 大鼠的胰腺和血浆胰岛素浓度：1）妊娠期，2）哺乳期，3）断奶后。单个胎儿样本来自两种交配类型：1）纯合肥胖雄性（fafa）与杂合雌性（Fafa），2）纯合瘦型雄性和雌性（FaFa）。来自相似交配的哺乳期和断奶后幼崽进行了部分胰腺切除术、尾静脉采血，并进行回顾性鉴定。与纯合瘦型胎儿相比，携带 fa 基因的 21 日龄胎儿血浆胰岛素浓度升高（P < 0.001），胰腺胰岛素浓度略低（P < 0.05）。在哺乳期，肥胖前期和瘦型幼崽的胰腺和血浆胰岛素浓度均无差异，只是肥胖前期幼崽在出生后第 20 天胰腺胰岛素浓度升高（P < 0.05）。断奶后 24 小时血浆胰岛素浓度升高（P < 0.01）。这些数据支持以下假设：fa 基因在妊娠期引发代谢变化，这些变化在哺乳期受到调节，但在断奶时再次出现。数据还表明，7 至 12 日龄肥胖前期幼崽脂肪细胞大小和脂蛋白脂肪酶活性的增加不依赖于同时存在的高胰岛素血症。
