Toledo-Pereyra L H, Gordon D A, MacKenzie G H
Am Surg. 1982 Jan;48(1):28-31.
Hepatocyte transplantation is one modality that has been suggested for providing temporary hepatic support during periods of liver regeneration following acute hepatic failure. Although liver cells have been successfully transplanted in the rat, transplantation in a larger model is necessary before these techniques can be applied clinically. This study attempts to define the immunologic response of the canine recipients to intrasplenic hepatocyte allografts. Our results indicate that canine hepatocytes are highly immunogenic, initiating a host response which destroyed the allograft within one week post-transplantation (survival range 1-6 days, mean +/- SD = 3.33 +/- 1.86 days). Administration of azathioprine post-transplantation significantly (P less than 0.01) extended the survival of the hepatocyte allografts (survival range 3-9 days, mean +/- SD = 5.66 +/- 2.16 days. However, long-term survival was not seen using this type of minimal immunosuppression. Histologic observation also confirmed these findings. Based on these results, therefore, caution is recommended prior to the application of hepatocyte transplantation to the clinical setting.
肝细胞移植是一种在急性肝衰竭后的肝再生期间提供临时肝脏支持的方法。虽然肝细胞已成功移植到大鼠体内，但在这些技术能够应用于临床之前，有必要在更大的模型中进行移植。本研究试图确定犬类受体对脾内肝细胞同种异体移植的免疫反应。我们的结果表明，犬肝细胞具有高度免疫原性，会引发宿主反应，在移植后一周内破坏同种异体移植物（存活范围1 - 6天，平均±标准差 = 3.33 ± 1.86天）。移植后给予硫唑嘌呤显著（P < 0.01）延长了肝细胞同种异体移植物的存活时间（存活范围3 - 9天，平均±标准差 = 5.66 ± 2.16天）。然而，使用这种最小程度的免疫抑制未观察到长期存活。组织学观察也证实了这些发现。因此，基于这些结果，在将肝细胞移植应用于临床之前建议谨慎行事。
