Laurent G, Gourdin M F, Reyes F
J Clin Pathol. 1982 Feb;35(2):139-43. doi: 10.1136/jcp.35.2.139.
Surface immunoglobulins (Ig) of normal and malignant lymphoid cells were detected on prefixed, smeared (method A) and live (method B) cell suspensions; the results were compared with regard to staining patterns, specificity and sensitivity. In both methods surface Ig were detected by a direct immunoperoxidase procedure using conjugated purified antibody. Although method A has practical advantages, method B is more sensitive. The reasons for this discrepancy are discussed in relation to surface Ig denaturation and redistribution.
在经固定、涂片的（方法A）以及活细胞悬液（方法B）中检测正常和恶性淋巴样细胞的表面免疫球蛋白（Ig）；从染色模式、特异性和敏感性方面对结果进行了比较。在两种方法中，均使用偶联的纯化抗体通过直接免疫过氧化物酶法检测表面Ig。虽然方法A具有实际优势，但方法B更为敏感。结合表面Ig变性和重新分布对这种差异的原因进行了讨论。
