人淋巴细胞表面免疫球蛋白的检测：使用直接免疫过氧化物酶法对活细胞和固定细胞的比较研究。
Detection of surface immunoglobulins of human lymphoid cells: a comparative study of live and fixed cells using a direct immunoperoxidase procedure.
作者信息
Laurent G, Gourdin M F, Reyes F
出版信息
J Clin Pathol. 1982 Feb;35(2):139-43. doi: 10.1136/jcp.35.2.139.
Abstract
Surface immunoglobulins (Ig) of normal and malignant lymphoid cells were detected on prefixed, smeared (method A) and live (method B) cell suspensions; the results were compared with regard to staining patterns, specificity and sensitivity. In both methods surface Ig were detected by a direct immunoperoxidase procedure using conjugated purified antibody. Although method A has practical advantages, method B is more sensitive. The reasons for this discrepancy are discussed in relation to surface Ig denaturation and redistribution.
摘要
在经固定、涂片的（方法A）以及活细胞悬液（方法B）中检测正常和恶性淋巴样细胞的表面免疫球蛋白（Ig）；从染色模式、特异性和敏感性方面对结果进行了比较。在两种方法中，均使用偶联的纯化抗体通过直接免疫过氧化物酶法检测表面Ig。虽然方法A具有实际优势，但方法B更为敏感。结合表面Ig变性和重新分布对这种差异的原因进行了讨论。
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本文引用的文献
1
Immunoperoxidase staining of surface and intracellular immunoglobulin in human neoplastic lymphoid cells.人肿瘤性淋巴细胞表面及细胞内免疫球蛋白的免疫过氧化物酶染色
J Clin Pathol. 1980 Jul;33(7):609-16. doi: 10.1136/jcp.33.7.609.
2
Immunoperoxidase detection of immunoglobulins in cells of immunoproliferative diseases. A comparison between conjugate and nonconjugate (PAP) procedures.免疫增殖性疾病细胞中免疫球蛋白的免疫过氧化物酶检测。共轭法与非共轭法（PAP法）的比较。
Am J Clin Pathol. 1980 Sep;74(3):265-74. doi: 10.1093/ajcp/74.3.265.
3
A direct lead method for the electron microscopic visualization of alkaline phosphatase activity.一种用于电子显微镜观察碱性磷酸酶活性的直接铅法。
J Histochem Cytochem. 1966 May;14(5):429-31. doi: 10.1177/14.5.429.
4
Immunoglobulins on the surface of lymphoid cells in Waldenström's macroglobulinemia.华氏巨球蛋白血症中淋巴样细胞表面的免疫球蛋白
J Clin Invest. 1972 Mar;51(3):701-5. doi: 10.1172/JCI106858.
5
Leukemic reticuloendotheliosis: presence of a receptor for cytophilic antibody.白血病性网状内皮增生症：嗜细胞性抗体受体的存在。
Am J Med. 1974 Jul;57(1):108-14. doi: 10.1016/0002-9343(74)90775-x.
6
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Prog Clin Immunol. 1974;2:121-74.
7
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Immunochemistry. 1971 Jan;8(1):1-6. doi: 10.1016/0019-2791(71)90416-2.
8
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