Buchegger F, Phan M, Rivier D, Carrel S, Accolla R S, Mach J P
J Immunol Methods. 1982 Mar 12;49(2):129-39. doi: 10.1016/0022-1759(82)90271-x.
A solid-phase enzyme immunoassay using both mouse monoclonal and goat polyclonal antibodies against carcinoembryonic antigen (CEA) was developed. The assay detects 0.6 to 1.2 ng of CEA per ml of serum and has 3 incubation steps which can be performed in 1 day. Polystyrene balls coated with polyclonal goat anti-CEA antibodies are first incubated with heat-extracted serum samples. Bound CEA is then detected by addition of mouse monoclonal antibodies, followed by goat IgG anti-mouse IgG1 coupled to alkaline phosphatase. Results with this enzyme immunoassay using monoclonal antibodies (M-EIA) have been compared with those obtained by the conventional inhibition radioimmunoassay (RIA) using goat antiserum. Three hundred and eighty serum samples from 167 patients with malignant or non-malignant diseases and from 134 normal individuals with or without heavy smoking habits were analyzed by the 2 assays. Excellent correlation between the results of the 2 assays was obtained, but the M-EIA, using monoclonal antibodies from a single hybridoma, did not discriminate better than the conventional RIA between CEA produced by different types of carcinoma and between CEA associated with malignant or non-malignant diseases. Follow-up studies of several patients by sequential CEA determinations with the 2 assays showed that the M-EIA was as accurate as the RIA for the detection of tumor recurrences.
开发了一种使用抗癌胚抗原（CEA）的小鼠单克隆抗体和山羊多克隆抗体的固相酶免疫测定法。该测定法可检测每毫升血清中0.6至1.2纳克的CEA，有3个孵育步骤，可在1天内完成。首先将包被有山羊抗CEA多克隆抗体的聚苯乙烯球与热提取的血清样本孵育。然后通过加入小鼠单克隆抗体检测结合的CEA，接着加入与碱性磷酸酶偶联的山羊抗小鼠IgG1。使用单克隆抗体的这种酶免疫测定法（M-EIA）的结果已与使用山羊抗血清的传统抑制放射免疫测定法（RIA）的结果进行了比较。对167例患有恶性或非恶性疾病的患者以及134例有或无重度吸烟习惯的正常个体的380份血清样本进行了这两种测定法的分析。两种测定法的结果之间具有极好的相关性，但使用来自单个杂交瘤的单克隆抗体的M-EIA在区分不同类型癌症产生的CEA以及与恶性或非恶性疾病相关的CEA方面并不比传统RIA更好。通过用这两种测定法对几名患者进行连续CEA测定的随访研究表明，M-EIA在检测肿瘤复发方面与RIA一样准确。
