Diller K R
J Microsc. 1982 Apr;126(Pt 1):9-28. doi: 10.1111/j.1365-2818.1982.tb00354.x.
A comprehensive review is presented of low temperature optical microscopy techniques as applied to the study of freezing processes in biological systems. Emphasis is placed on analysis of physical and physiological parameters which were measured and/or controlled and the procedures for effecting such operations. Quantitative analysis of photomicrographs by digital computer processing is also discussed.
本文对应用于生物系统冷冻过程研究的低温光学显微镜技术进行了全面综述。重点在于分析所测量和/或控制的物理和生理参数,以及进行此类操作的程序。还讨论了通过数字计算机处理对显微照片进行定量分析的方法。