Vossepoel A M, Smeulders A W, van den Broek K
Comput Programs Biomed. 1979 Dec;10(3):231-44. doi: 10.1016/0010-468x(79)90072-2.
A computer program is described for delineation and measurement of microscopical objects, such as cells and chromosomes, which may have been scanned using absorbance, fluorescence or reflectance microscopy. The quality of the object delineation is optimized through the controur ratio, which is simply computed from the object contour. Geometrical features, like the perimeter and area are computed. A new definition is introduced for the background region, especially suited for the analysis of closely packed objects. This definition is based upon a comparison between total staining material content values resulting from a variety of methods and circumstances. The program is principally intended for use in the on-line real-time environment of a small laboratory computer, but may be used off-line as well. It has facilities for displaying the results and the process by which they are produced. A program module is described for displaying scan data on a bilevel display using an adaptation of the sigma-delta method.
描述了一种计算机程序，用于描绘和测量微观物体，如细胞和染色体，这些物体可能已通过吸光度、荧光或反射显微镜进行了扫描。通过轮廓比优化物体描绘的质量，轮廓比可简单地根据物体轮廓计算得出。计算诸如周长和面积等几何特征。引入了一种适用于背景区域的新定义，特别适合对紧密堆积物体的分析。该定义基于对各种方法和情况产生的总染色物质含量值的比较。该程序主要旨在用于小型实验室计算机的在线实时环境，但也可离线使用。它具备显示结果及其产生过程的功能。描述了一个程序模块，用于通过对sigma-delta方法的改编在双电平显示器上显示扫描数据。
