Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Room 208, Norman, OK 73019, USA.
Antimicrob Agents Chemother. 2010 Dec;54(12):5366-8. doi: 10.1128/AAC.00719-10. Epub 2010 Sep 27.
We investigated possible cross talk between endogenous antioxidants glutathione, spermidine, and glutathionylspermidine and drug efflux in Escherichia coli. We found that cells lacking either spermidine or glutathione are less susceptible than the wild type to novobiocin and certain aminoglycosides. In contrast, exogenous glutathione protects against both bactericidal and bacteriostatic antibiotics. The glutathione protection does not require the AcrAB efflux pump but fails in cells lacking TolC because exogenous glutathione is toxic to these cells.
我们研究了内源性抗氧化剂谷胱甘肽、亚精胺和谷胱甘肽亚精胺与大肠杆菌药物外排之间可能存在的串扰。我们发现，缺乏亚精胺或谷胱甘肽的细胞比野生型细胞对新生霉素和某些氨基糖苷类药物的敏感性降低。相比之下，外源性谷胱甘肽可以保护细胞免受杀菌和抑菌抗生素的影响。谷胱甘肽的保护作用不需要 AcrAB 外排泵，但在缺乏 TolC 的细胞中失败，因为外源性谷胱甘肽对这些细胞有毒。
