Choe J, Kolodrubetz D, Grunstein M
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1484-7. doi: 10.1073/pnas.79.5.1484.
The sequences of the two histones H2A genes in the yeast Saccharomyces cerevisiae have been determined. These genes encode two histone H2A subtypes which are 131 amino acids in length but differ at 2 amino acid positions: an Ala leads to Thr and a Thr leads to Ala change at positions 124 and 125. Thus, the two histone H2A subtypes have identical amino acid compositions. The coding regions of the two H2A genes are homologous at 369 of 393 bases (94%), with all but 2 of the 24 changes being silent. There is only 30% homology in the 5' flanking sequences of the two H2A genes. Like other eukaryotic histone genes, the yeast H2A genes are not interrupted by intervening sequences. When the yeast H2A histones are compared to those from other eukaryotes, there is at least 80% homology in amino acid sequence.
已确定酿酒酵母中两个组蛋白H2A基因的序列。这些基因编码两种组蛋白H2A亚型，其长度为131个氨基酸，但在两个氨基酸位置存在差异：在第124和125位，一个丙氨酸变为苏氨酸，一个苏氨酸变为丙氨酸。因此，这两种组蛋白H2A亚型具有相同的氨基酸组成。两个H2A基因的编码区在393个碱基中的369个处同源（94%），24个变化中除2个外其余均为沉默突变。两个H2A基因的5'侧翼序列仅有30%的同源性。与其他真核生物组蛋白基因一样，酵母H2A基因不被间隔序列打断。当将酵母H2A组蛋白与其他真核生物的组蛋白进行比较时，氨基酸序列至少有80%的同源性。
