Hood M A, Ness G E
Appl Environ Microbiol. 1982 Mar;43(3):578-84. doi: 10.1128/aem.43.3.578-584.1982.
In in vitro estuarine water and sediment chambers, the survival of Vibrio cholerae and Escherichia coli was determined by plate counting and direct counting techniques. V. cholerae strains included environmental, clinical, and serotype O1 and non-O1 isolates, whereas E. coli strains included ATCC 25922 and a freshly cultured human isolate. Recovery of V. cholerae varied significantly with incubation temperature. Growth and extended periods of survival occurred in sterile sediments, sterile waters, and nonsterile waters, but not in nonsterile sediments. In contrast to V. cholerae, viable cells of E. coli decreased rapidly in both sterile and nonsterile estuarine waters. Direct counts revealed that E. coli cells were intact in the estuarine water, but attempts to resuscitate them were unsuccessful. The data suggest that V. cholerae survives better in estuarine waters than E. coli. The results may explain the recent observations that V. cholerae levels do not correlate well with fecal coliform concentrations in estuarine waters. Furthermore, the results add increasing evidence to support the theory that V. cholerae is an autochthonous bacterium in estuaries.
在体外河口海水和沉积物培养箱中，通过平板计数和直接计数技术测定霍乱弧菌和大肠杆菌的存活率。霍乱弧菌菌株包括环境菌株、临床菌株以及O1血清型和非O1血清型分离株，而大肠杆菌菌株包括ATCC 25922和一株新培养的人体分离株。霍乱弧菌的回收率随培养温度的变化而显著不同。在无菌沉积物、无菌海水和非无菌海水中，霍乱弧菌均能生长并延长存活期，但在非无菌沉积物中则不能。与霍乱弧菌不同，大肠杆菌的活细胞在无菌和非无菌河口海水中均迅速减少。直接计数显示，大肠杆菌细胞在河口海水中保持完整，但复苏这些细胞的尝试未成功。数据表明，霍乱弧菌在河口海水中的存活能力比大肠杆菌更强。这些结果可能解释了最近的观察结果，即河口海水中霍乱弧菌的水平与粪大肠菌群浓度的相关性不佳。此外，这些结果为支持霍乱弧菌是河口本地细菌这一理论提供了越来越多的证据。
