Blackwell Karen Dyer, Oliver James D
Department of Biology, University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
J Microbiol. 2008 Apr;46(2):146-53. doi: 10.1007/s12275-007-0216-2. Epub 2008 Jun 11.
While numerous studies have characterized the distribution and/or ecology of various pathogenic Vibrio spp., here we have simultaneously examined several estuarine sites for Vibrio vulnificus, V. cholerae, and V. parahaemolyticus. For a one year period, waters and sediment were monitored for the presence of these three pathogens at six different sites on the east coast of North Carolina in the United States. All three pathogens, identified using colony hybridization and PCR methods, occurred in these estuarine environments, although V. cholerae occurred only infrequently and at very low levels. Seventeen chemical, physical, and biological parameters were investigated, including salinity, water temperature, turbidity, dissolved oxygen, levels of various inorganic nutrients and dissolved organic carbon, as well as total vibrios, total coliforms, and E. coli. We found each of the Vibrio spp. in water and sediment to correlate to several of these environmental measurements, with water temperature and total Vibrio levels correlating highly (P<0.0001) with occurrence of the three pathogens. Thus, these two parameters may represent simple assays for characterizing the potential public health hazard of estuarine waters.
尽管众多研究已对各种致病性弧菌属的分布和/或生态特征进行了描述，但在此我们同时在美国北卡罗来纳州东海岸的几个河口位点对创伤弧菌、霍乱弧菌和副溶血性弧菌进行了检测。在为期一年的时间里，对美国北卡罗来纳州东海岸六个不同位点的水体和沉积物进行监测，以检测这三种病原体的存在情况。使用菌落杂交和PCR方法鉴定出，所有这三种病原体均存在于这些河口环境中，不过霍乱弧菌出现的频率很低且含量也非常少。对17种化学、物理和生物学参数进行了调查，包括盐度、水温、浊度、溶解氧、各种无机养分和溶解有机碳的含量，以及总弧菌数、总大肠菌群数和大肠杆菌数。我们发现水中和沉积物中的每种弧菌属均与其中几项环境测量值相关，水温与总弧菌数水平与这三种病原体的出现高度相关（P<0.0001）。因此，这两个参数可能代表了用于表征河口水体潜在公共卫生危害的简单检测方法。
