Loftager M K, Høier-Madsen M, Koch C, Andersen V
Department of Medicine TTA, State University Hospital, Copenhagen, Denmark.
Clin Exp Immunol. 1988 Nov;74(2):242-6.
A murine hybridoma cell line (aDNA35I9) secreting anti-DNA antibodies was used as a model for haemolytic plaque forming cells in an assay where DNA-conjugated sheep red blood cells (DNA-SRBC) were used as target cells. DEAE-dextran, employed to abolish the anti-complementary activity of agar gel, completely inhibited anti-DNA plaques. This problem was solved by using agarose instead of agar. Since the occurrence of small plaques may make reading of the test difficult, it was established that plaque size could be increased by decreasing the antigen density on the target cells. Free DNA in the gel inhibited plaque formation, indicating the specificity of the assay. Spleen cells from mice of the strains MRL/MP and NZB/W which are known to develop a stage of autoimmunity, produced plaques in numbers which were correlated to the age of the mice and to the anti-DNA antibody level in serum.
一种分泌抗DNA抗体的小鼠杂交瘤细胞系（aDNA35I9）被用作溶血空斑形成细胞的模型，在该实验中，用DNA偶联的绵羊红细胞（DNA-SRBC）作为靶细胞。用于消除琼脂凝胶抗补体活性的DEAE-葡聚糖完全抑制了抗DNA空斑。通过使用琼脂糖代替琼脂解决了这个问题。由于小空斑的出现可能会使试验读数困难，因此确定可以通过降低靶细胞上的抗原密度来增加空斑大小。凝胶中的游离DNA抑制空斑形成，表明该实验具有特异性。已知会发展到自身免疫阶段的MRL/MP和NZB/W品系小鼠的脾细胞产生的空斑数量与小鼠年龄和血清中的抗DNA抗体水平相关。
