Ridley M J, Russell D F, Ridley D S
Int J Lepr Other Mycobact Dis. 1982 Mar;50(1):11-9.
The immunoperoxidase technique was used to assess the quantity and situation of various immunological factors in 24 skin biopsies which represented the leprosy spectrum from TT to LL. The factors were immunoglobulins (IgG and IgM), complement components (C3, C3d and Clq), plasminogen, muramidase (lysozyme), C-reactive protein and alpha-1-antitrypsin. The results were compared with previous reports on the assessment of these factors in serum. The quantities of these factors in the lesions produced peaks at TT and LL, with a dip in the BT-BB region (C-reactive protein and alpha-1-antitrypsin excepted). The immunoglobulins, present mainly in plasma cells and lymphocytes, correlated in general with reports of serum levels. The complement components were present in appreciable amounts, though the serum levels are depressed; they were seen in young mononuclear cells with a low bacterial load. All factors produced an ascending gradient in active lesions from BT to BL or LL, which correlated with the bacterial load and its viability. In regression (studied only in LL) there was a decrease in all factors. In TT there was an increase in most factors which did not correlate with the antigen load, and which probably resulted in an excess of antibody over antigen. In active LL there is probably an antigen excess. The results suggest the possibility that there is a common defect from BT to LL, in which the generation of immunological factors within the lesion of immunological factors within the lesion is a secondary response to the antigenic load. In TT alone (a rare group) is there an enhanced immunological response unrelated to the antigen load. In support of this was the finding of Ia antigen only in TT lesions.
采用免疫过氧化物酶技术评估了24份皮肤活检标本中各种免疫因子的数量和情况，这些标本代表了从结核样型（TT）到瘤型（LL）的麻风病谱。这些因子包括免疫球蛋白（IgG和IgM）、补体成分（C3、C3d和Clq）、纤溶酶原、溶菌酶、C反应蛋白和α1抗胰蛋白酶。将结果与先前关于血清中这些因子评估的报告进行了比较。这些因子在病变中的数量在TT和LL处达到峰值，在界线类偏结核样型（BT）-界线类偏瘤型（BB）区域出现下降（C反应蛋白和α1抗胰蛋白酶除外）。主要存在于浆细胞和淋巴细胞中的免疫球蛋白，总体上与血清水平的报告相关。补体成分含量可观，尽管血清水平降低；在细菌载量低的年轻单核细胞中可见。所有因子在从BT到BL或LL的活动性病变中呈现上升梯度，这与细菌载量及其活力相关。在退行期（仅在LL中研究）所有因子都减少。在TT中，大多数因子增加，这与抗原载量无关，可能导致抗体超过抗原。在活动性LL中可能存在抗原过剩。结果表明，从BT到LL可能存在共同缺陷，其中病变内免疫因子的产生是对抗原负荷的继发反应。仅在TT（一个罕见组）中存在与抗原负荷无关的增强免疫反应。支持这一点的是仅在TT病变中发现Ia抗原。
