Bannikov G A, Guelstein V I, Montesano R, Tint I S, Tomatis L, Troyanovsky S M, Vasiliev J M
J Cell Sci. 1982 Apr;54:47-67. doi: 10.1242/jcs.54.1.47.
Morphological changes associated with neoplastic transformation of epithelial cells were studied in a series of IAR cell lines derived from rat liver. The series included three independently obtained, non-tumorigenic lines and five derived, tumorigenic lines. The morphology of cell surfaces was observed by scanning electron microscopy; the distribution of actin, tubulin and fibronectin was determined by indirect immunofluorescence. All the non-tumorigenic lines had a typical epithelioid morphology: isolated cells of these lines spread on the substratum had a discoid shape and contained circular, marginal bundles of microfilaments and microtubules. In denser areas, the cells formed monolayered sheets with characteristic marginal bundles of microfilaments near the free edges. Decreased spreading of isolated cells on the substratum was the characteristic feature that distinguished tumorigenic lines from their non-tumorigenic parent lines. In particular a decrease in the size of the ring-like, peripheral lamella and its disintegration into several discrete lamellar zones were often observed; as a result, the cell shape was altered from discoid to polygonal or elongated. The altered distribution of microfilament bundles and microtubules was characteristic in elongated cells; the pattern of the cytoskeletal elements of these cells resembled that of polarized fibroblasts. Complete disappearance of microfilament bundles was observed in cells of only one tumorigenic line. Various degrees of disorganization of monolayered cell sheets were observed in tumorigenic cultures, accompanied by an altered distribution of microfilament bundles. The alterations in the fibronectin-containing structures were more complex: there were often fewer fibronectin "spots' and fibrils at the lower surfaces of cells of tumorigenic cultures as compared with those of non-tumorigenic ones; there were more fibrils in dense cultures of certain lines but fewer in others. It is concluded that alterations in the ability to spread on the substratum and to form cell-cell contacts are common features of morphologically transformed fibroblastic and epithelial cultures. However, the actual changes in the cytoskeletal structures that accompany these alterations are different in transformed cultures of various tissue types.
在一系列源自大鼠肝脏的IAR细胞系中，研究了与上皮细胞肿瘤转化相关的形态学变化。该系列包括三个独立获得的非致瘤性细胞系和五个衍生的致瘤性细胞系。通过扫描电子显微镜观察细胞表面的形态；通过间接免疫荧光确定肌动蛋白、微管蛋白和纤连蛋白的分布。所有非致瘤性细胞系都具有典型的上皮样形态：这些细胞系中分散在基质上的单个细胞呈盘状，含有圆形的边缘微丝束和微管束。在较密集的区域，细胞形成单层片，在自由边缘附近有特征性的边缘微丝束。分离的细胞在基质上的铺展减少是区分致瘤性细胞系与其非致瘤性亲代细胞系的特征性特征。特别是，经常观察到环状外周薄片的尺寸减小并分解为几个离散的薄片区域；结果，细胞形状从盘状变为多边形或细长形。微丝束和微管束的分布改变在细长细胞中具有特征性；这些细胞的细胞骨架元件模式类似于极化的成纤维细胞。仅在一个致瘤性细胞系的细胞中观察到微丝束完全消失。在致瘤性培养物中观察到单层细胞片的不同程度的紊乱，伴随着微丝束分布的改变。含纤连蛋白结构的改变更为复杂：与非致瘤性培养物相比，致瘤性培养物细胞下表面的纤连蛋白“斑点”和纤维通常较少；某些细胞系的密集培养物中有更多的纤维，但其他细胞系中较少。得出的结论是，在基质上铺展和形成细胞 - 细胞接触的能力改变是形态学转化的成纤维细胞和上皮细胞培养物的共同特征。然而，在各种组织类型的转化培养物中，伴随这些改变的细胞骨架结构的实际变化是不同的。
