Swierenga S H, Goyette R, Marceau N
Exp Cell Res. 1984 Jul;153(1):39-49. doi: 10.1016/0014-4827(84)90446-4.
Normal rat liver T51B epithelial cells and Morris no. 7795 hepatoma cells growing exponentially were exposed for 24 h to standard medium containing low (0.02 mM) calcium, a concentration which drastically reduces the proliferation of normal but not tumour cells. Cell surface morphology was examined by scanning electron microscopy (SEM); and the distribution and organization of microtubules, cytokeratin and vimentin filaments, and microfilaments were analysed by indirect immunofluorescence microscopy using specific antibodies. Calcium deprivation caused the loss of intercellular cohesion in both cell types and the appearance of some microvilli and blebs, particularly on tumour cells. However, marked differential (normal vs tumour cells) effects on the organizational integrity of the cytoskeleton fibrillar network were observed. Extracellular calcium deprivation led to a particular rearrangement of microtubules, and a perinuclear accumulation of cytokeratin and vimentin filaments in normal, but not in tumour cells. A massive concentration of actin-containing microfilaments was observed in the cell periphery and blebs of hepatoma cells. In the light of the possible involvement of calcium in controlling cytoskeleton assembly, the differing cytoskeletal changes of the two cell types may be linked to their different proliferative capabilities in low-calcium medium.
将指数生长的正常大鼠肝T51B上皮细胞和莫里斯7795号肝癌细胞暴露于含有低钙（0.02 mM）的标准培养基中24小时，该浓度会显著降低正常细胞而非肿瘤细胞的增殖。通过扫描电子显微镜（SEM）检查细胞表面形态；并使用特异性抗体通过间接免疫荧光显微镜分析微管、细胞角蛋白和波形蛋白丝以及微丝的分布和组织。钙剥夺导致两种细胞类型的细胞间黏附丧失，并出现一些微绒毛和泡状突起，特别是在肿瘤细胞上。然而，观察到对细胞骨架纤维网络的组织完整性有明显的差异（正常细胞与肿瘤细胞）影响。细胞外钙剥夺导致微管的特定重排，以及正常细胞而非肿瘤细胞中细胞角蛋白和波形蛋白丝在核周积累。在肝癌细胞的细胞周边和泡状突起中观察到大量含肌动蛋白的微丝聚集。鉴于钙可能参与控制细胞骨架组装，两种细胞类型不同的细胞骨架变化可能与其在低钙培养基中的不同增殖能力有关。
