Lemmon V, Gottlieb D I
J Neurosci. 1982 May;2(5):531-5. doi: 10.1523/JNEUROSCI.02-05-00531.1982.
Five monoclonal antibodies were selected by immunizing mice with embryonic chick optic nerve and screening the resultant hybridoma antibodies by immunofluorescence on sections of the retina. The optic nerve was chosen as the immunizing tissue since it is presumably enriched in ganglion cell-specific antigens. These antibodies bind to antigens which are largely localized to the inner part of the retina. Two of the antibodies, RET1 and RET2, show highly selective binding to the optic fiber layer. Another, RET3, stains the optic fiber layer, ganglion cell layer, and inner plexiform layer. Antibody RET4 stains the optic fiber layer, ganglion cell layer, inner plexiform layer, and the inner one-half of the inner nuclear layer; this antibody binds to an antigen on or associated with the plasma membrane. Antibody RET5 stains the retinal ganglion cell layer faintly and the inner and outer plexiform layers strongly. The potential utility of these antibodies for future studies on retinal development is discussed.
用鸡胚视神经免疫小鼠，并通过视网膜切片免疫荧光筛选所得杂交瘤抗体，从而选出了五种单克隆抗体。选择视神经作为免疫组织是因为它可能富含神经节细胞特异性抗原。这些抗体与主要定位于视网膜内部的抗原结合。其中两种抗体RET1和RET2对视神经纤维层表现出高度选择性结合。另一种抗体RET3可使视神经纤维层、神经节细胞层和内网状层染色。抗体RET4可使视神经纤维层、神经节细胞层、内网状层以及内核层的内半部染色；该抗体与质膜上或与质膜相关的一种抗原结合。抗体RET5使视网膜神经节细胞层染色较淡，而使内、外网状层染色较强。本文讨论了这些抗体在未来视网膜发育研究中的潜在用途。
