Grunwald G B, Fredman P, Magnani J L, Trisler D, Ginsburg V, Nirenberg M
Proc Natl Acad Sci U S A. 1985 Jun;82(12):4008-12. doi: 10.1073/pnas.82.12.4008.
Mouse monoclonal antibody 18B8 detects developmentally regulated antigens in chicken retina and brain. The antigens detected by immunofluorescence appear initially on cell bodies in retinas of 6-13 day embryos. In older embryos during synapse formation and in adults, the antigen is localized in discrete laminae within the inner synaptic layer of retina and also is present in the outer synaptic layer and the outer segments of photoreceptor cells. The antigens from retina and brain were purified partially and were shown to be gangliosides of unknown structure that contain at least two sialic acid residues. Gangliosides that are recognized by antibody 18B8 change both qualitatively and quantitatively during neuronal development. These changes were correlated with the spatial and temporal changes in antigen expression detected histochemically.
小鼠单克隆抗体18B8可检测鸡视网膜和大脑中发育调控的抗原。通过免疫荧光检测到的抗原最初出现在6至13天胚胎视网膜的细胞体上。在突触形成期的 older embryos 以及成体中，该抗原定位于视网膜内突触层的离散板层中，并且也存在于外突触层和光感受器细胞的外段。视网膜和大脑中的抗原被部分纯化，并显示为结构未知的神经节苷脂，其含有至少两个唾液酸残基。被抗体18B8识别的神经节苷脂在神经元发育过程中在质量和数量上都会发生变化。这些变化与通过组织化学检测到的抗原表达的时空变化相关。
