Drazba J, Pierce M, Lemmon V
Department of Neurobiology, Anatomy and Cell Science, University of Pittsburgh School of Medicine, Pennsylvania 15261.
Dev Biol. 1991 May;145(1):154-63. doi: 10.1016/0012-1606(91)90221-n.
A monoclonal antibody, Mab 8A2, that recognizes a novel set of gangliosides was produced by immunizing a mouse with Embryonic Day 14 chick optic nerve. Immunohistochemical studies of the developing chick retina revealed a complex pattern of Mab 8A2 immunoreactivity. Initially, staining is concentrated in the optic fiber layer in the central retina. Later in development, the most intense staining is seen at the periphery of the retina and 8A2 immunoreactivity appears in other retina layers. In the adult retina, 8A2 immunoreactivity is lost from the optic fiber layer but persists in the inner plexiform layer, inner nuclear layer, and outer plexiform layer. Cell culture experiments showed intense staining of neurites from retinal ganglion cells but no staining of Muller cells. Biochemical characterization of the epitope recognized by Mab 8A2 suggests that it includes a 9-O-acetyl group that is present on five different gangliosides. The 8A2 immunoreactive gangliosides are distinct from and have slower mobilities on thin-layer chromatographs than those recognized by Mab D1.1 which recognizes 9-O-acetyl GD3.
通过用胚胎第14天的鸡视神经免疫小鼠，制备了一种识别一组新型神经节苷脂的单克隆抗体Mab 8A2。对发育中的鸡视网膜进行免疫组织化学研究，发现Mab 8A2免疫反应呈现出复杂的模式。最初，染色集中在视网膜中央的视神经纤维层。在发育后期，视网膜周边出现最强的染色，并且8A2免疫反应出现在视网膜的其他层。在成年视网膜中，8A2免疫反应从视神经纤维层消失，但在内网状层、内核层和外网状层持续存在。细胞培养实验显示视网膜神经节细胞的神经突有强烈染色，但穆勒细胞无染色。对Mab 8A2识别的表位进行生化表征表明，它包括存在于五种不同神经节苷脂上的9-O-乙酰基。8A2免疫反应性神经节苷脂与识别9-O-乙酰基GD3的Mab D1.1所识别的神经节苷脂不同，并且在薄层色谱上的迁移率较慢。
