Russell M H, Engel E, Vaughn W K, McGee B J
J Cell Sci. 1977 Jun;25:59-71. doi: 10.1242/jcs.25.1.59.
Hybrids between A9 (HGPRT-) and B82 (TK-), mouse heteroploid fibroblast lines, were obtained through continuous cultivation and clonal selection; such hybrids showed marked segregation and by conventional stains displayed chromosome numbers and distribution similar to that of either parental type. Detailed analyses by Giemsa (G)- and centromeric-banding of these parental lines, and of 4 of the reduced hybrids, maintained in culture for up to 5 years, revealed the following points: (1) The distribution of the majority of individual chromosomal classes was similar for 3 of the hybrid cell lines. (2) Over two-thirds of the chromosomal arms in both the parental lines and hybrid lines were identical to normal mouse telocentric chromosomes. (3) For 2 of the hybrid lines, segregation was particularly marked with respect to those chromosomal arms whose G-banding patterns were identical to the wild-type genome; this indicated that segregation had occurred at the expense of redundant chromosomal material introduced by cell fusion. These banded studies demonstrated that segregation chiefly accounted for the sharp reduction in chromosome numbers while recombination accounted for the chromosome heterogeneity of the hybrid cells as compared to the parental genomes.
通过连续培养和克隆选择获得了A9（次黄嘌呤鸟嘌呤磷酸核糖转移酶缺陷型）和B82（胸苷激酶缺陷型）小鼠异倍体成纤维细胞系之间的杂种；此类杂种显示出明显的分离现象，并且通过常规染色显示其染色体数目和分布与任一亲本类型相似。对这些亲本系以及在培养中保存长达5年的4个减数杂种进行吉姆萨（G）显带和着丝粒显带的详细分析，揭示了以下几点：（1）3个杂种细胞系中大多数单个染色体类别的分布相似。（2）亲本系和杂种系中超过三分之二的染色体臂与正常小鼠端着丝粒染色体相同。（3）对于2个杂种系，就那些G显带模式与野生型基因组相同的染色体臂而言，分离现象尤为明显；这表明分离是以细胞融合引入的多余染色体物质为代价发生的。这些显带研究表明，分离主要导致了染色体数目的急剧减少，而重组则导致了杂种细胞与亲本基因组相比的染色体异质性。
