Medrano L, Phalente L, Buttin G
Cell Biol Int Rep. 1979 Sep;3(6):503-14. doi: 10.1016/0309-1651(79)90086-9.
Hoechst 33258 fluorescent staining can be coupled with G-banding to identify the chromosomal contribution of each parent in mouse-rabbit hybridomas. A fast and essentially complete segregation of rabbit chromosomes is obtained in these cells. The rabbit X chromosome is preferentially maintained in media imposing HGPRT activity for cell growth. Mouse-rabbit hybridomas, some of which secrete rabbit immunoglobulin chains, should be a convenient material for the identification of chromosomes governing rabbit Ig chain synthesis.
Hoechst 33258荧光染色可与G带分析相结合，以确定小鼠 - 兔杂交瘤中每个亲本的染色体贡献。在这些细胞中可快速且基本完全地分离兔染色体。在施加HGPRT活性以促进细胞生长的培养基中，兔X染色体优先得以保留。小鼠 - 兔杂交瘤（其中一些分泌兔免疫球蛋白链）应是鉴定控制兔Ig链合成的染色体的便利材料。
