Figurski D H, Pohlman R F, Bechhofer D H, Prince A S, Kelton C A
Proc Natl Acad Sci U S A. 1982 Mar;79(6):1935-9. doi: 10.1073/pnas.79.6.1935.
Cloning of specific regions of RK2, a broad host range incompatibility group P plasmid, has revealed three genes: kilA, kilB, and kilC. Each of these genes can cause loss of viability of an Escherichia coli host. This effect on the host is normally prevented by the functions of three additional RK2 genes: korA, korB, and korC. Each kor gene is specific for a particular kil gene. The kil and kor genes are located in four distinct regions of the RK2 genome. The three kil genes are not clustered and, with the possible exception of kilA, they are also well separated from their corresponding kor genes. We have found that the korA and korB determinants are not peculiar to RK2 but instead are highly conserved throughout the incompatibility group P plasmids.
RK2是一种广宿主范围的不相容群P质粒，对其特定区域的克隆揭示了三个基因：kilA、kilB和kilC。这些基因中的每一个都可导致大肠杆菌宿主丧失活力。宿主的这种效应通常会被另外三个RK2基因（korA、korB和korC）的功能所阻止。每个kor基因对特定的kil基因具有特异性。kil和kor基因位于RK2基因组的四个不同区域。三个kil基因并不成簇，除了kilA可能例外，它们与其相应的kor基因也相距甚远。我们发现，korA和korB决定簇并非RK2所特有，而是在整个不相容群P质粒中高度保守。
