广宿主范围质粒RK2的kilA基因的调控：korA、korB和一个新基因korE的作用
Control of the kilA gene of the broad-host-range plasmid RK2: involvement of korA, korB, and a new gene, korE.
作者信息
Young C, Burlage R S, Figurski D H
出版信息
J Bacteriol. 1987 Mar;169(3):1315-20. doi: 10.1128/jb.169.3.1315-1320.1987.
Abstract
Broad-host-range plasmid RK2 encodes several different kil genes which are potentially lethal to an Escherichia coli host. The kil genes and the essential RK2 replication gene trfA are regulated by the products of kor genes. We have shown previously that kilA can be controlled by a constitutively expressed korA gene. In this study, we have found that the wild-type, autoregulated korA gene is insufficient for control of kilA cloned on high-copy-number plasmids. One of two other genes must also be present with korA. One gene is korB, originally discovered by its ability to control the determinants in the kilB region and later found to affect expression of both trfA and korA. The other is a new gene, korE, which has been cloned from the 2.2' to 4.1' region located between korC and kilA. Studies with a kilA-cat fusion suggest that korA, korB, and korE all participate in the control of kilA gene expression.
摘要
广宿主范围质粒RK2编码几种不同的kil基因，这些基因对大肠杆菌宿主可能具有致死性。kil基因和必需的RK2复制基因trfA受kor基因产物的调控。我们之前已经表明，kilA可以由组成型表达的korA基因控制。在本研究中，我们发现野生型、自我调节的korA基因不足以控制克隆在高拷贝数质粒上的kilA。另外两个基因中的一个也必须与korA同时存在。一个基因是korB，最初因其控制kilB区域决定因素的能力而被发现，后来发现它会影响trfA和korA的表达。另一个是新基因korE，它是从位于korC和kilA之间的2.2'至4.1'区域克隆而来的。对kilA-cat融合体的研究表明，korA、korB和korE都参与了kilA基因表达的调控。
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本文引用的文献
1
Broad host range plasmid RK2 encodes multiple kil genes potentially lethal to Escherichia coli host cells.广宿主范围质粒RK2编码多个对大肠杆菌宿主细胞有潜在致死性的kil基因。
Proc Natl Acad Sci U S A. 1982 Mar;79(6):1935-9. doi: 10.1073/pnas.79.6.1935.
2
The korB gene of broad host range plasmid RK2 is a major copy number control element which may act together with trfB by limiting trfA expression.广宿主范围质粒RK2的korB基因是一个主要的拷贝数控制元件，它可能通过限制trfA的表达与trfB共同发挥作用。
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Nucleotide sequence of the trfA gene of broad host-range plasmid RK2.广宿主范围质粒RK2的trfA基因的核苷酸序列。
J Mol Biol. 1984 May 25;175(3):251-62. doi: 10.1016/0022-2836(84)90347-4.
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Proteins encoded by the trans-acting replication and maintenance regions of broad host range plasmid RK2.广宿主范围质粒RK2的反式作用复制和维持区域所编码的蛋白质。
Plasmid. 1984 Jan;11(1):48-57. doi: 10.1016/0147-619x(84)90006-4.
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Gene regulation in plasmid RK2: positive control by korA in the expression of korC.质粒RK2中的基因调控：korA对korC表达的正调控
J Bacteriol. 1984 Jan;157(1):247-52. doi: 10.1128/jb.157.1.247-252.1984.
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Replication of derivatives of the broad host range plasmid RK2 in two distantly related bacteria.广宿主范围质粒RK2的衍生物在两种亲缘关系较远的细菌中的复制
Plasmid. 1983 May;9(3):325-30. doi: 10.1016/0147-619x(83)90010-0.
7
The trfA and trfB promoter regions of broad host range plasmid RK2 share common potential regulatory sequences.广宿主范围质粒RK2的trfA和trfB启动子区域共享共同的潜在调控序列。
Nucleic Acids Res. 1984 Apr 25;12(8):3619-30. doi: 10.1093/nar/12.8.3619.
8
Analysis of the trfA region of broad host-range plasmid RK2 by transposon mutagenesis and identification of polypeptide products.通过转座子诱变对广宿主范围质粒RK2的trfA区域进行分析并鉴定多肽产物。
J Mol Biol. 1984 May 25;175(3):229-49. doi: 10.1016/0022-2836(84)90346-2.
9
Control of cell division by sex factor F in Escherichia coli. I. The 42.84-43.6 F segment couples cell division of the host bacteria with replication of plasmid DNA.大肠杆菌中性别因子F对细胞分裂的控制。I. 42.84 - 43.6 F片段将宿主细菌的细胞分裂与质粒DNA的复制联系起来。
J Mol Biol. 1984 Apr 25;174(4):605-25. doi: 10.1016/0022-2836(84)90086-x.
10
Map location and nucleotide sequence of korA, a key regulatory gene of promiscuous plasmid RK2.乱交性质粒RK2的关键调控基因korA的图谱定位及核苷酸序列
Nucleic Acids Res. 1983 Nov 11;11(21):7453-69. doi: 10.1093/nar/11.21.7453.
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