Sterling R J, Sharp P J
Cell Tissue Res. 1982;222(2):283-98. doi: 10.1007/BF00213213.
Nerve fibers and perikarya containing LH-RH-like material were identified immunohistochemically in the diencephalon of the domestic hen using the peroxidase-anti-peroxidase technique. Perikarya were thinly scattered in bilateral bands close to the third ventricle extending from the nucleus praeopticus paraventricularis magnocellularis, passing in front of the anterior commissure into the septal area. In this latter area, the perikarya tended to spread out laterally. A few perikarya were seen in the anterior portion of the nucleus paraventricularis magnocellularis but were not found in the infundibular nuclear complex. Fibre tracts were seen running dorso-ventrally in the preoptic area apparently associated with the lamina terminalis. Fibres, possibly nerve terminals, were found in the lamina terminalis and in the external layers of the anterior and posterior divisions of the median eminence. A large number of fibres was seen distributed throughout the infundibular nuclear complex; scattered fibres were found close to the third ventricle in the anterior hypothalamus. Extrahypothalamic fibers were also observed to project from the septal area into other parts of the telencephalon.
运用过氧化物酶抗过氧化物酶技术，在家鸡间脑中通过免疫组织化学方法鉴定出含有促黄体生成激素释放激素（LH-RH）样物质的神经纤维和神经细胞体。神经细胞体稀疏地散布在靠近第三脑室的双侧带中，从视前室旁大细胞核延伸，经过前连合前方进入隔区。在隔区，神经细胞体倾向于向外侧扩散。在视前室旁大细胞核的前部可见少数神经细胞体，但在漏斗核复合体中未发现。在视前区可见纤维束背腹向走行，显然与终板有关。在终板以及正中隆起前后部的外层发现有纤维，可能是神经末梢。在整个漏斗核复合体中可见大量纤维分布；在下丘脑前部靠近第三脑室处发现有散在纤维。还观察到隔区有下丘脑外纤维投射到端脑的其他部位。
