Buschard K, Madsbad S, Krarup T, Rygaard J
Clin Exp Immunol. 1982 Apr;48(1):189-95.
Suppressor cell activity (SCA) in patients with insulin-dependent diabetes mellitus (IDDM), has been found depressed at diagnosis and normal in the remission period. To investigate whether poor metabolic control as seen at diagnosis influences SCA, two groups of patients in poor glycaemic control were examined. Group 1 consisted of nine patients with IDDM of 27±4 months, with a mean glucosuria of 84±19 (s.e.m.) g/24 hr. Group 2 comprised eight patients with newly diagnosed IDDM, examined for the first time 4±1 days after diagnosis, when having a mean glucosuria of 43±14 g/24 hr. After 1 week of hospitalization with strict glycaemic control resulting in a massive reduction of the glucosuria (group 1 now showing 0±0 g/24 hr, group 2, 16±5 g/24 hr), the patients were re-examined. SCA was investigated for the third time after approximately 4 weeks (group 1) and after 5 months (group 2), when the patients were outpatients (glucosuria: group 1, 61±20 g/24 hr; group 2, 17±6 g/24 hr). The suppressive effect of lymphocytes was investigated after incubation with concanavalin A followed by inactivation. Suppression was measured as the ability of the lymphocytes to inhibit H-thymidine incorporation in concanavalin A-stimulated normal donor lymphocytes. There were no significant differences in SCA between the first (group 1,25±7; group 2, 22±11) and the second (group 1, 22±8; group 2, 15±5) examination. At the third examination, group 1 patients displayed 35±5, and group 2 patients, now being in the remission period, 44±4. Only the third value of group 2 patients was significantly higher than the second value (<0·01). SCA of healthy controls were 35±3. It is concluded that short-term effects on SCA of improvement of the glycaemic control are unlikely, at least in patients without severe ketoacidosis. On the other hand, late effects on SCA of good metabolic control, after weeks or months, could not be excluded.
胰岛素依赖型糖尿病（IDDM）患者的抑制细胞活性（SCA）在诊断时被发现降低，而在缓解期则正常。为了研究诊断时所见的代谢控制不佳是否会影响SCA，对两组血糖控制不佳的患者进行了检查。第一组由9例病程为27±4个月的IDDM患者组成，平均糖尿量为84±19（标准误）g/24小时。第二组包括8例新诊断的IDDM患者，在诊断后4±1天首次检查，此时平均糖尿量为43±14 g/24小时。经过1周严格的血糖控制住院治疗，导致糖尿量大幅减少（第一组现在显示为0±0 g/24小时，第二组为16±5 g/24小时）后，对患者进行了重新检查。在大约4周后（第一组）和5个月后（第二组）对患者进行了第三次SCA检查，此时患者为门诊患者（糖尿量：第一组，61±20 g/24小时；第二组，17±6 g/24小时）。在用伴刀豆球蛋白A孵育然后灭活后，研究淋巴细胞的抑制作用。抑制作用以淋巴细胞抑制伴刀豆球蛋白A刺激的正常供体淋巴细胞中H-胸腺嘧啶核苷掺入的能力来衡量。第一次（第一组，25±7；第二组，22±11）和第二次（第一组，22±8；第二组，15±5）检查之间的SCA没有显著差异。在第三次检查时，第一组患者显示为35±5，而处于缓解期的第二组患者为44±4。只有第二组患者的第三个值显著高于第二个值（<0.01）。健康对照组的SCA为35±3。得出的结论是，至少在没有严重酮症酸中毒的患者中，血糖控制改善对SCA的短期影响不太可能。另一方面，数周或数月后良好代谢控制对SCA的后期影响不能排除。
