一种细胞表面单克隆抗体（H366）有助于区分人类细胞毒性T细胞和抑制性T细胞。
A cell surface monoclonal antibody (H366) helps to discriminate human cytotoxic from suppressor T cells.
作者信息
Al-Sakkaf L, Pozzilli P, Sensi M, Irving W L, Bottazzo G F
出版信息
Clin Exp Immunol. 1985 Dec;62(3):594-9.
A study has been undertaken to differentiate T cytotoxic (Tc) and T suppressor (Ts) cell subsets using a monoclonal antibody termed H366 (mouse IgG2b) previously reported to phenotype natural killer and killer (NK/K) cells. Mononuclear cell suspensions from 14 normal subjects were depleted of H366+ cells by means of complement dependent cytotoxicity and the remaining cells were phenotyped with CD8 and CD4 monoclonal antibodies. The effects of depletion with H366 plus complement (C1) on the induction and activity of suppressor and cytotoxic T cells was also examined. The results indicate that H366 antibody recognizes in addition to NK/K cells, a population of Tc but not Ts or helper cells. Therefore, H366 antibody can be useful for obtaining Ts enriched lymphocyte subpopulations and this property may also be used for the enumeration of suppressor cells in the peripheral blood in disease states.
一项研究利用一种名为H366（小鼠IgG2b）的单克隆抗体来区分细胞毒性T细胞（Tc）和抑制性T细胞（Ts）亚群，该抗体先前被报道可对自然杀伤细胞和杀伤细胞（NK/K）进行表型分析。通过补体依赖性细胞毒性去除14名正常受试者的单核细胞悬液中的H366+细胞，并用CD8和CD4单克隆抗体对剩余细胞进行表型分析。还检测了用H366加补体（C1）去除细胞对抑制性和细胞毒性T细胞的诱导和活性的影响。结果表明，H366抗体除了识别NK/K细胞外，还识别一群Tc细胞，但不识别Ts细胞或辅助性T细胞。因此，H366抗体可用于获得富含Ts细胞的淋巴细胞亚群，这一特性也可用于疾病状态下外周血中抑制性T细胞的计数。
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