仓鼠对麻疹病毒实验性中枢神经系统感染的免疫反应。

Immune response of hamsters to experimental central nervous system infection with measles virus.

作者信息

Tobler L H, Johnson K P, Buehring G C

出版信息

J Neuroimmunol. 1982 Jun;2(3-4):307-20. doi: 10.1016/0165-5728(82)90063-7.

Abstract

Forty-two 21-day-old Syrian golden hamsters were inoculated intracerebrally with a hamster-adapted Mantooth strain of measles virus. For the duration of the experiment, the animals demonstrated measles virus infection of the central nervous system documented by histology and detection of measles antigen by fluorescent antibody staining. The lymphocyte-rosetting response to infection in the hamster was monitored for 25 days after inoculation utilizing a human cell line (HEp-2) persistently infected with the hamster-adapted measles virus. The rosetting response reached a peak on the third day after virus inoculation and then declined to a constant low level by the tenth day. This decline corresponded to the peak of the humoral immune response as measured by the measles hemagglutination-inhibition titer. The rosetting response-appears in this model to be a response to the acute phase of infection.

摘要

42只21日龄的叙利亚金黄地鼠经脑内接种了适应仓鼠的曼图斯麻疹病毒株。在实验期间，通过组织学和荧光抗体染色检测麻疹抗原，证明这些动物的中枢神经系统受到麻疹病毒感染。接种后25天内，利用持续感染适应仓鼠的麻疹病毒的人细胞系（HEp-2）监测仓鼠对感染的淋巴细胞玫瑰花结反应。玫瑰花结反应在病毒接种后第三天达到峰值，然后在第十天降至持续的低水平。这种下降与通过麻疹血凝抑制效价测量的体液免疫反应峰值相对应。在这个模型中，玫瑰花结反应似乎是对感染急性期的反应。