Rauch J, Murphy E, Roths J B, Stollar B D, Schwartz R S
J Immunol. 1982 Jul;129(1):236-41.
Anti-idiotypic antibodies were used to analyze relatedness of anti-DNA autoantibodies of MRL mice. The antiidiotypic antibodies, produced by immunization of rabbits with MRL-Ipr-derived hybridoma anti-DNA antibodies, were defined by their ability to inhibit specifically the binding of hybridoma anti-DNA antibody to DNA. One monoclonal anti-DNA antibody, H130, cross-reacted with two thirds of other hybridoma anti-DNA antibodies. The H130/anti-H130 system detected high amounts (up to 5 mg/ml) of immunoreactivity in sera of MRL-Ipr mice, whereas levels in MRL-+ sera were relatively low. The H130 family increased 10-fold in MRL-Ipr mice over the course of 5 mo, but its levels did not correlate chronologically with those of anti-DNA antibodies, cardiolipin-binding antibodies, or total serum immunoglobulins. H130 cross-reactivity was found, by quantitative immunoadsorption, in 40 to 60% of serum anti-DNA antibodies; however, a large excess of H130-related serum antibodies did not bind to DNA. The H130 family and anti-DNA antibodies therefore seem to constitute overlapping populations.
抗独特型抗体被用于分析MRL小鼠抗DNA自身抗体的相关性。用源自MRL-Ipr的杂交瘤抗DNA抗体免疫兔子所产生的抗独特型抗体，通过其特异性抑制杂交瘤抗DNA抗体与DNA结合的能力来定义。一种单克隆抗DNA抗体H130与三分之二的其他杂交瘤抗DNA抗体发生交叉反应。H130/抗H130系统在MRL-Ipr小鼠血清中检测到大量（高达5毫克/毫升）的免疫反应性，而MRL-+血清中的水平相对较低。在5个月的时间里，H130家族在MRL-Ipr小鼠中增加了10倍，但其水平与抗DNA抗体、心磷脂结合抗体或总血清免疫球蛋白的水平在时间上并无关联。通过定量免疫吸附发现，40%至60%的血清抗DNA抗体存在H130交叉反应；然而，大量过量的与H130相关的血清抗体并不与DNA结合。因此，H130家族和抗DNA抗体似乎构成了重叠群体。
