Sibley J T, Latimer L J, Lee J S
Department of Medicine, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
J Immunol. 1988 May 15;140(10):3502-7.
Hed 10 is a ssDNA-specific mAb derived from an NZB/W autoimmune mouse. ADP 1 is a poly(ADP-ribose)-specific mAb derived from a C57BL/6 mouse. Rabbit anti-idiotypic sera were raised against Hed 10 and ADP 1. By affinity chromatography it was shown that at least 20 to 30% of DNA-binding antibodies contained these idiotypes. The sera were also used to evaluate idiotypic cross-reactivity among 26 diverse, predominantly anti-nucleic acid, murine mAb. Each serum bound directly to several mAb and in addition inhibited the binding of several antibodies to their appropriate Ag. The anti-Hed 10 serum detected an idiotype which was restricted to antibodies that bound to poly(dT) and/or poly(ADP-ribose). The anti-ADP 1 serum detected a more widely distributed idiotype contained in antibodies which bound to a variety of nucleic acids. Although both sera bound directly to Hed 10, only the anti-Hed 10 serum could compete for the binding of Hed 10 to poly(dT). On the other hand, both sera could compete for the binding of ADP 1 to poly(ADP-ribose) as well as bind directly to ADP 1. In addition anti-ADP 1 serum appeared to enhance, rather than inhibit, the binding of one mAb to native calf thymus DNA and poly[d(AT)] but had no effect on the binding to several ssDNA. These results demonstrate that many antibodies which recognize DNA and poly(ADP-ribose) have shared idiotypes. This may be of relevance to the development of autoimmunity because poly(ADP-ribose) is ubiquitous and immunogenic.
Hed 10是一种源自NZB/W自身免疫小鼠的单链DNA特异性单克隆抗体。ADP 1是一种源自C57BL/6小鼠的聚（ADP-核糖）特异性单克隆抗体。制备了针对Hed 10和ADP 1的兔抗独特型血清。通过亲和层析表明，至少20%至30%的DNA结合抗体含有这些独特型。这些血清还用于评估26种不同的、主要是抗核酸的鼠单克隆抗体之间的独特型交叉反应性。每种血清都直接与几种单克隆抗体结合，此外还抑制几种抗体与其相应抗原的结合。抗Hed 10血清检测到一种独特型，该独特型仅限于与聚（dT）和/或聚（ADP-核糖）结合的抗体。抗ADP 1血清检测到一种分布更广泛的独特型，存在于与多种核酸结合的抗体中。虽然两种血清都直接与Hed 10结合，但只有抗Hed 10血清能竞争Hed 10与聚（dT）的结合。另一方面，两种血清都能竞争ADP 1与聚（ADP-核糖）的结合，也能直接与ADP 1结合。此外，抗ADP 1血清似乎增强而非抑制一种单克隆抗体与天然小牛胸腺DNA和聚[d（AT）]的结合，但对与几种单链DNA的结合没有影响。这些结果表明，许多识别DNA和聚（ADP-核糖）的抗体具有共同的独特型。这可能与自身免疫的发展有关，因为聚（ADP-核糖）普遍存在且具有免疫原性。
