在补充有胎牛血清或马血清的培养物中，刺激中国仓鼠淋巴细胞的不同最佳PHA浓度。

Different optimal PHA concentrations for stimulation of Chinese hamster lymphocytes in cultures supplemented with foetal calf serum or horse serum.

作者信息

De Jong B, Anders G J, Van der Meer I H, Zijlstra J, Idenburg V J

出版信息

J Immunol Methods. 1982;50(1):11-5. doi: 10.1016/0022-1759(82)90299-x.

Abstract

Chinese hamster lymphocyte cultures were supplemented with foetal calf serum (FCS) or hose serum (HS). Addition of 2-mercaptoethanol (2-ME) to cultures supplemented with these sera resulted in a marked increase lymphocyte proliferation. The PHA concentrations necessary for optimal lymphocyte stimulation were much lower in cultures supplemented with HS than with FCS. Phytohaemagglutinin (PHA) concentrations optimal for stimulation of a given number of lymphocytes in cultures supplemented with FCS often gave inhibition of similar cultures supplemented with HS.

摘要

中国仓鼠淋巴细胞培养物中添加了胎牛血清（FCS）或马血清（HS）。在添加这些血清的培养物中加入2-巯基乙醇（2-ME）会导致淋巴细胞增殖显著增加。在添加HS的培养物中，实现最佳淋巴细胞刺激所需的PHA浓度远低于添加FCS的培养物。在添加FCS的培养物中，刺激给定数量淋巴细胞的最佳植物血凝素（PHA）浓度，在添加HS的类似培养物中往往会产生抑制作用。