de Jong B, van der Meer I H, Zijlstra J, Anders G J
J Immunol Methods. 1976;12(1-2):91-102. doi: 10.1016/0022-1759(76)90099-5.
A microtechnique for the culture of Chinese Hamster lymphocytes is described using Cooke microtiter plates with 100,000 leucocytes in a culture volume of 0.1 ml and a culture time of three days. The culture media used were RPMI 1640 and Trowell T8 supplemented with 20% foetal calf serum (FCS) and 2 mu PHA. The cells were harvested with a Skatron cell culture harvester using glass fibre filters. Various technical aspects of the lymphocyte cultures from the Chinese Hamster are described. The relevance of changes in culture conditions to the variability of culture results was analysed for PHA and FCS concentrations, different culture media, cell concentration, vessel shape and culture duration.
描述了一种用于培养中国仓鼠淋巴细胞的微量技术，使用库克微量滴定板，培养体积为0.1毫升，含100,000个白细胞，培养时间为三天。使用的培养基是补充有20%胎牛血清（FCS）和2微克PHA的RPMI 1640和特罗威尔T8。使用玻璃纤维滤器通过Skatron细胞培养收获器收获细胞。描述了来自中国仓鼠的淋巴细胞培养的各种技术方面。分析了培养条件的变化与PHA和FCS浓度、不同培养基、细胞浓度、容器形状和培养持续时间等培养结果变异性的相关性。
