血清/PHA比例、微孔板孔形状和2-巯基乙醇对中国仓鼠淋巴细胞培养中不同数量细胞刺激的影响。
The influence of the serum/PHA ratio, microplate well shape and 2-mercaptoethanol on the stimulation of different numbers of cells in lymphocyte cultures from the chinese hamster.
作者信息
de Jong B, Anders G J, Zijlstra J, van Der Meer I H
出版信息
J Immunol Methods. 1977;16(1):59-72. doi: 10.1016/0022-1759(77)90039-4.
PMID:864264
Abstract
Conditions for microculture of Chinese hamster lymphocytes are described which allow measurement of thymidine uptake with 6000 to 1000 lymphocytes per culture. The relationship between degree of cell stimulation, PHA concentration culture surface and cell concentration is described, as well as the influence of addition of 2-mercaptoethanol to the cultures.
摘要
本文描述了中国仓鼠淋巴细胞微培养的条件，该条件允许在每个培养物中用6000至1000个淋巴细胞测量胸苷摄取量。文中描述了细胞刺激程度、PHA浓度、培养表面和细胞浓度之间的关系，以及向培养物中添加2-巯基乙醇的影响。
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