一种制备经典途径（C1q）和替代途径（因子D）缺失的人血清的一步法。
A one-step procedure for preparation of classical pathway (C1q) and alternative pathway (factor D) depleted human serum.
作者信息
Praz F, Roque Antunes Barreira M C, Lesavre P
出版信息
J Immunol Methods. 1982;50(2):227-31. doi: 10.1016/0022-1759(82)90229-0.
PMID:7045233
Abstract
A simple method for preparation of a serum depleted in both C1q and Factor D is described. The hemolytic activities of both pathways are completely abolished and can be fully restored using the respective purified complement components. Furthermore, this serum is useful for studying cell systems since blocking either complement pathway does not require chelating agents.
摘要
本文描述了一种制备同时缺乏C1q和D因子的血清的简单方法。两条途径的溶血活性均被完全消除，并且使用各自纯化的补体成分可使其完全恢复。此外，这种血清对于研究细胞系统很有用，因为阻断任一补体途径都不需要螯合剂。
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