Padrão G R, Malamud D R, Panek A D, Mattoon J R
Mol Gen Genet. 1982;185(2):255-61. doi: 10.1007/BF00330795.
The recessive, nuclear gene mutation glc1, which causes glycogen deficiency in Saccharomyces cerevisiae, is highly pleiotropic. Studies of the inheritance of glc1 revealed two classes of phenotypic characteristics: I. Traits invariably associated with the mutant gene and II. Traits whose expressions require the presence of glc1 and one or more additional genes. Class I traits include glycogen deficiency and the loss of capacity to accumulate trehalose in nonproliferating conditions. Traits in the second class include a decreased rate of growth on ethanol medium, a deficiency in cytochrome a.a3 and an enhanced accumulation of pigment, probably a metalloporphyrin. Constructed strains containing both glc1 and the constitutive maltose fermentation gene MAL4c can accumulate trehalose but not glycogen during growth on glucose. However, accumulated trehalose is degraded when cells are exposed to nonproliferating conditions. It is proposed that the glc1 mutation affects a regulatory system, probably involving a protein kinase and/or protein phosphatase, which regulates glycogen synthase and trehalase. Independent regulation of trehalose synthesis by a system controlled by MAL4c is indicated.
隐性核基因突变glc1会导致酿酒酵母出现糖原缺乏，该突变具有高度多效性。对glc1遗传的研究揭示了两类表型特征：I. 总是与突变基因相关的性状；II. 其表达需要glc1和一个或多个其他基因存在的性状。第一类性状包括糖原缺乏以及在非增殖条件下积累海藻糖能力的丧失。第二类性状包括在乙醇培养基上生长速率降低、细胞色素a.a3缺乏以及色素（可能是金属卟啉）积累增加。构建的同时含有glc1和组成型麦芽糖发酵基因MAL4c的菌株在葡萄糖上生长时能够积累海藻糖但不能积累糖原。然而，当细胞暴露于非增殖条件时，积累的海藻糖会被降解。有人提出，glc1突变影响一种调节系统，可能涉及一种蛋白激酶和/或蛋白磷酸酶，该系统调节糖原合酶和海藻糖酶。这表明由MAL4c控制的系统对海藻糖合成具有独立调节作用。
