Ferreira J C, Panek A D
Depto. de Bioquimica, Universidade Federal do Rio de Janeiro, Brasil.
Biochem Mol Biol Int. 1993 Dec;31(6):1081-90.
S. cerevisiae has at least two enzymes for trehalose synthesis. In this paper we report some characteristics of the ADPG-dependent trehalose-6-phosphate synthase. As opposed to the UDPG-dependent enzyme, the ADPG-dependent activity did not respond to a glucose signal nor to a heat shock. Mutant strains, deficient in the UDPG-dependent enzyme but possessing the ADPG-dependent activity, did not accumulate trehalose either during growth or during sporulation. A diploid, homozygous for the deficiency in the UDPG-dependent synthase activity, was constructed. During sporulation the ADPG-dependent activity remained constant. However, when mutant spores were subjected to a glucose signal, trehalose was accumulated to the same extent as in control spores.
酿酒酵母至少有两种用于海藻糖合成的酶。在本文中，我们报道了依赖ADP-葡萄糖的海藻糖-6-磷酸合酶的一些特性。与依赖UDP-葡萄糖的酶不同，依赖ADP-葡萄糖的活性对葡萄糖信号或热激均无反应。缺乏依赖UDP-葡萄糖的酶但具有依赖ADP-葡萄糖活性的突变菌株，在生长或孢子形成过程中均不积累海藻糖。构建了一个依赖UDP-葡萄糖合酶活性缺陷的纯合二倍体。在孢子形成过程中，依赖ADP-葡萄糖的活性保持恒定。然而，当突变孢子受到葡萄糖信号刺激时，海藻糖的积累程度与对照孢子相同。
