Saunders A M
J Histochem Cytochem. 1977 Aug;25(8):1001-12. doi: 10.1177/25.8.70461.
The development of an automated differential white blood cell counter is reviewed. After the red cells have been lysed, the white cells are counted by staining and passing through an electro-optical chamber in liquid suspension, surrounded by a laminar, or sheath, stream. Staining procedures were made specific for each type of leukocyte, and separate channels were used for counting each type. Staining intensity and characteristics of the various types of blood cells are discussed. They relate to enzyme levels and the effect on differentiation and identification of the cells. Since reasons for some of the design features are not obvious, discussion of the relevant problems is included. Several applications that go beyond routine differential counting are described.
本文综述了自动白细胞分类计数器的发展。红细胞裂解后，白细胞通过染色并在液体悬浮液中通过一个光电计数室进行计数，该计数室被层流或鞘流包围。针对每种白细胞类型制定了特定的染色程序，并使用单独的通道对每种类型进行计数。讨论了各类血细胞的染色强度和特征。它们与酶水平以及对细胞分化和识别的影响有关。由于某些设计特点的原因并不明显，因此也包括了对相关问题的讨论。还描述了一些超出常规分类计数的应用。
