Minde T E, Vallanger M, Haneberg B, Sandberg S
Department of Pediatrics, Haukeland Hospital, Bergen, Norway.
Eur J Haematol Suppl. 1990;53:54-6. doi: 10.1111/j.1600-0609.1990.tb01529.x.
A method for automated differential leukocyte counting using peroxidase staining and light scatter (H1) failed to present results in more than 25% of blood samples from newborn infants. These failures were mostly seen at or below 2 weeks of age, and with high concentrations of haemoglobin F (HbF). Dilution of the blood with equal amounts of isotonic saline made the problem disappear almost completely. The results of differential counts on such diluted blood samples also compared more favourably with traditional manual counts of blood smears than did the results obtained with undiluted blood. The present method with blood diluted 1/2 is therefore recommended for routine use in newborn infants.
一种使用过氧化物酶染色和光散射进行白细胞自动分类计数的方法（H1），在超过25%的新生儿血样中未能得出结果。这些失败情况大多出现在2周龄及以下的婴儿，以及血红蛋白F（HbF）浓度较高的样本中。用等量的等渗盐水稀释血液后，这个问题几乎完全消失。与未稀释血液的检测结果相比，对这种稀释血样进行分类计数的结果与传统血涂片手工计数结果的匹配度更高。因此，建议采用血液稀释1/2的现行方法用于新生儿的常规检测。
