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酿酒酵母呼吸缺陷型突变体的流动微量热法。

Flow microcalorimetry of a respiration-deficient mutant of Saccharomyces cerevisiae.

作者信息

Loureiro-Dias M C, Arrabaça J D

出版信息

Z Allg Mikrobiol. 1982;22(2):119-22.

PMID:7046269
Abstract

In aerobic batch cultures in mineral medium with glucose of a respiration-deficient mutant of Saccharomyces cerevisiae, growth parameters were estimated and the heat evolved was measured by a flow microcalorimeter. A growth enthalpy of -163.6 joule per mole of glucose consumed was measured. Under anaerobic conditions, the value was -134.6 joule, closer to the expected for alcoholic fermentation alone. The difference was found to be due to cyanide-resistant respiration under aerobic conditions.

摘要

在以葡萄糖为碳源的矿物培养基中,对酿酒酵母呼吸缺陷型突变体进行好氧分批培养,估算其生长参数,并通过流动式微量量热计测量热量释放。测得每消耗1摩尔葡萄糖的生长焓为-163.6焦耳。在厌氧条件下,该值为-134.6焦耳,更接近仅酒精发酵的预期值。发现这种差异是由于好氧条件下存在抗氰呼吸。

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