Jacobson L, Halmann M, Yariv J
Biochem J. 1982 Mar 1;201(3):473-9. doi: 10.1042/bj2010473.
The volutin granule was isolated from yeast by disruption of freeze-dried cells in an organic solvent and density-gradient-gradient centrifugation. The granule is composed of two types of macromolecule, a linear-chain polyphosphate and four basic proteins, of molecular weights ranging from 10 000 to 20 000. In the dissolved granule these macromolecules are in a complex that is uniform by hydrodynamic criteria (s20,w = 22.3 S). The polyphosphate separated from this complex gives a single 31P n.m.r. resonance and in the analytical ultracentrifuge behaves as a monodisperse solute of molecular weight 245 000 +/- 1000. In the 31P n.m.r. spectrum of yeast used for its isolation, this polyphosphate accounts for 14% of total cell polyphosphate.
通过在有机溶剂中冻干细胞并进行密度梯度离心，从酵母中分离出异染粒。该颗粒由两种大分子组成，一种是线性链状多磷酸盐，还有四种碱性蛋白质，分子量在10000至20000之间。在溶解的颗粒中，这些大分子形成一种复合物，根据流体动力学标准，该复合物是均匀的（沉降系数s20,w = 22.3 S）。从该复合物中分离出的多磷酸盐给出单一的31P核磁共振共振信号，在分析超速离心机中表现为分子量为245000±1000的单分散溶质。在用于分离异染粒的酵母的31P核磁共振谱中，这种多磷酸盐占细胞总多磷酸盐的14%。
