肝组织小块。一种用于研究小块组织代谢的简单、快速且可重复的方法，应用于大鼠肝脏的葡萄糖醛酸化。
Liver snips. A simple, rapid and reproducible method for studying metabolism in small fragments of tissue, as applied to glucuronidation in rat liver.
作者信息
Pollard M R, Dutton G J
出版信息
Biochem J. 1982 Feb 15;202(2):469-73. doi: 10.1042/bj2020469.
Abstract
A simple, rapid method is described of preparing intact cells as small (about 2mm) pieces of organized tissue capable of performing synthetic metabolic functions. It has been applied to the study of glucuronidation in rat liver. In this process, snips appear less damaged, more versatile and more active than tissue slices and yield results of reproducibility comparable with those with homogenates. From a comparison with the literature, snips glucuronidate the substrates employed at a rate much the same as in perfused preparations and some 30% less than the rate in isolated-hepatocyte suspensions; the advantages they offer in certain situations over these two techniques are discussed.
摘要
本文描述了一种简单、快速的方法，可将完整细胞制备成能够执行合成代谢功能的小（约2毫米）片有组织的组织。该方法已应用于大鼠肝脏中葡萄糖醛酸化的研究。在此过程中，与组织切片相比，剪碎组织看起来损伤更小、用途更广且活性更高，并且产生的可重复性结果与匀浆相当。通过与文献比较，剪碎组织对所用底物进行葡萄糖醛酸化的速率与灌注制剂中的速率大致相同，比分离肝细胞悬液中的速率低约30%；讨论了它们在某些情况下相对于这两种技术所具有的优势。
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Liver snips. A simple, rapid and reproducible method for studying metabolism in small fragments of tissue, as applied to glucuronidation in rat liver.肝组织小块。一种用于研究小块组织代谢的简单、快速且可重复的方法，应用于大鼠肝脏的葡萄糖醛酸化。
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本文引用的文献
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2
Decreased UDP-glucuronic acid in rat liver after ether narcosis: an isotachophoretic study.乙醚麻醉后大鼠肝脏中UDP-葡萄糖醛酸减少：等速电泳研究
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Assays for UDPglucuronyltransferase activities.UDP葡糖醛酸基转移酶活性测定
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Eur J Biochem. 1970 Dec;17(2):246-53. doi: 10.1111/j.1432-1033.1970.tb01160.x.
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Measurement of uridine diphosphate glucuronic acid concentrations and synthesis in animal tissues.动物组织中尿苷二磷酸葡萄糖醛酸浓度的测定及合成
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