Kawaciuková J, Dvoráková K, Potuzník V
Ustav lékarské mikrobiologie 2. lékarské fakulty UK, Praha-Motol.
Cesk Epidemiol Mikrobiol Imunol. 1991 May;40(3):170-6.
The author described the estimation of toxoplasmic antibodies by the method of reverse enzyme immunoanalysis (IgM-ELISA) in 476 sera. The results of IgM-ELISA were compared with the complement fixation reaction (CFR) and indirect fluorescent reaction IgM-ELISA. Of 217 sera which were IgM-NFR positive 115 were positive in IgM-ELISA. In clinically confirmed acute toxoplasmosis high absorbance values of IgM-ELISA were recorded and during repeated examination a dynamic drop of antibodies was observed. The author discusses the importance of IgM-ELISA for the differentiation of acute and chronic stages of toxoplasmosis and draw attention to false IgM-NFR positivity in the sera they examined.
作者描述了用反向酶免疫分析法(IgM - ELISA)对476份血清中的弓形虫抗体进行检测。将IgM - ELISA的结果与补体结合反应(CFR)及间接荧光反应IgM - ELISA进行了比较。在217份IgM - NFR呈阳性的血清中,有115份在IgM - ELISA中呈阳性。在临床确诊的急性弓形虫病中,记录到IgM - ELISA的高吸光度值,且在重复检测过程中观察到抗体呈动态下降。作者讨论了IgM - ELISA在区分弓形虫病急性和慢性阶段中的重要性,并提请注意他们所检测血清中IgM - NFR的假阳性情况。