Von Hoff D D, Forseth B, Metelmann H R, Harris G, Rowan S, Coltman C A
Cancer. 1982 Aug 15;50(4):696-701. doi: 10.1002/1097-0142(19820815)50:4<696::aid-cncr2820500413>3.0.co;2-0.
An in vitro soft agar technique was used to culture human malignant melanoma cells from 61 solid tumors, 17 lymph nodes, 11 effusions, and four bone marrow specimens from 93 patients with malignant melanoma. Colonies grew in soft agar from 64 (69%) of the 93 specimens. Fifty-five percent of the specimens cultured formed greater than or equal to 30 colonies per 500,000 nucleated cells plated. Light microscopy, electron microscopy, tumor marker, and athymic nude mouse studies provided evidence the colonies were composed of malignant melanoma cells. Drug sensitivity studies utilizing the cloning technique showed similarities between in vitro results and the general clinical experience noted with the same drugs. The human tumor cloning system represents a new model for future basic biology and clinical studies of human malignant melanoma.
采用体外软琼脂技术培养来自93例恶性黑色素瘤患者的61份实体瘤、17份淋巴结、11份积液和4份骨髓标本中的人恶性黑色素瘤细胞。93份标本中有64份（69%）在软琼脂中形成了集落。所培养的标本中有55%每接种500,000个有核细胞形成大于或等于30个集落。光学显微镜、电子显微镜、肿瘤标志物及无胸腺裸鼠研究均证实这些集落由恶性黑色素瘤细胞构成。利用克隆技术进行的药敏研究显示体外结果与使用相同药物的一般临床经验之间具有相似性。人肿瘤克隆系统为未来人类恶性黑色素瘤的基础生物学和临床研究提供了一种新模型。
