Sandbach J, Von Hoff D D, Clark G, Cruz A B, Obrien M
Cancer. 1982 Oct 1;50(7):1315-21. doi: 10.1002/1097-0142(19821001)50:7<1315::aid-cncr2820500717>3.0.co;2-7.
A human tumor cloning system has been utilized to grow human breast carcinoma. A total of 225 specimens have been placed in culture. One hundred thirty-two were from primary chest cancer specimens and 93 were from metastatic lesions. Of these, 71% of the primary breast carcinomas and 75% of metastases formed greater than or equal to 5 colonies per 500,000 cells plated. Forty-five percent of the primary breast carcinomas and 52% of the metastases formed enough colonies (greater than or equal to 30 colonies per 500,000 cells plated) to perform meaningful in vitro drug testing. Estrogen receptor status did not influence the percentage of tumors which formed colonies in vitro. Histologic and nude mouse studies provided confirmatory evidence the colonies were composed of breast cancer cells. In 176 in vitro chemotherapeutic drug tests, the anticancer agents commonly used clinically for treatment of breast cancer, i.e., Adriamycin, 5-fluorouracil, etc., showed some in vitro activity. This activity was not as dramatic as is seen in the clinic with these conventional agents. Future work should concentrate on improving the number of colonies which form from breast cancer specimens and on prospective use of the system for screening for new agents for the treatment of human breast cancer.
一种人类肿瘤克隆系统已被用于培养人类乳腺癌。总共225个标本被置于培养中。其中132个来自原发性胸癌标本，93个来自转移性病变。在这些标本中，71%的原发性乳腺癌和75%的转移灶每接种500,000个细胞形成大于或等于5个集落。45%的原发性乳腺癌和52%的转移灶形成了足够数量的集落（每接种500,000个细胞大于或等于30个集落），以便进行有意义的体外药物测试。雌激素受体状态并不影响体外形成集落的肿瘤百分比。组织学和裸鼠研究提供了确凿证据，证明这些集落由乳腺癌细胞组成。在176次体外化疗药物测试中，临床上常用于治疗乳腺癌的抗癌药物，即阿霉素、5-氟尿嘧啶等，显示出一些体外活性。这种活性不如这些传统药物在临床上所见的那样显著。未来的工作应集中于提高乳腺癌标本形成的集落数量，以及前瞻性地利用该系统筛选治疗人类乳腺癌的新药物。
