Bentley S A, Knutsen T, Whang-Peng J
Exp Hematol. 1982 Apr;10(4):367-72.
Marrow-derived adherent cells (MDAC) have been shown to provide a microenvironment which supports the proliferation of hematopoietic stem cells in continuous bone marrow culture. A study was undertaken to investigate the origin of MDAC. Normal CBA mice were transplanted with marrow cells from CBA donors bearing two T6 chromosomes. Five weeks after transplantation, the mice were sacrificed, their marrow cells explanted in liquid culture and the numbers of T6 positive and T6 negative mitoses were monitored during cultivation of MDAC. After 6 weeks, established MDAC monolayers were recharged with second explants of marrow cells and their capacity to support HSC proliferation in continuous marrow culture was assessed. The number of T6 positive mitoses declined steadily during the period of MDAC cultivation and none were detected after 4 weeks. The MDAC monolayers were able to support in vitro proliferation of CFUs for periods of at least 5 weeks. These results suggest that the in vitro hematopoietic microenvironment was of recipient origin and therefore stromal.
骨髓来源的贴壁细胞（MDAC）已被证明能提供一种微环境，在连续骨髓培养中支持造血干细胞的增殖。开展了一项研究以调查MDAC的起源。将带有两条T6染色体的CBA供体的骨髓细胞移植到正常CBA小鼠体内。移植后五周，处死小鼠，取出其骨髓细胞进行液体培养，并在MDAC培养过程中监测T6阳性和T6阴性有丝分裂的数量。六周后，用骨髓细胞的第二次外植体对已建立的MDAC单层进行再接种，并评估其在连续骨髓培养中支持造血干细胞增殖的能力。在MDAC培养期间，T6阳性有丝分裂的数量稳步下降，4周后未检测到。MDAC单层能够在至少5周的时间内支持集落形成单位的体外增殖。这些结果表明，体外造血微环境起源于受体，因此是基质性的。
