Wilkinson P C
Immunobiology. 1982 Apr;161(3-4):376-84. doi: 10.1016/S0171-2985(82)80095-8.
Locomotion of mouse macrophages in relation to spores of Candida albicans in mouse serum was studied by time-lapse cinematography using thioglycollate-elicited peritoneal macrophages, resident peritoneal macrophages, and a murine macrophage line (J774 B10). Thioglycollate-elicited macrophages and the cell line responded well to the spores, but very few of the resident macrophages showed any response. The macrophages showed chemotactic responses with straight-line locomotion towards spores. Thioglycollate-elicited macrophages, which were frequently spread on glass, could also respond to spores 20-30 micrometers away by chemotropism, i.e. extension of a large hyaline veil to engulf the spore without prior displacement of the body of the cell. Engulfed spores were then pulled into the organelle-rich cell centre. In this population, there was no incompatibility between spread morphology and motile behaviour. In contrast, J774 B10 did not spread and moved with a rounded morphology and with a very small anterior hyaline veil towards the gradient source. Macrophages moved more slowly than peripheral blood neutrophils and monocytes. The mean speed of thioglycollate-elicited and cell line macrophages was 3-4 micrometers per minute. The few motile resident peritoneal macrophages moved even more slowly, i.e. ca 2 micrometers per minute.
利用巯基乙酸盐诱导的腹腔巨噬细胞、常驻腹腔巨噬细胞和小鼠巨噬细胞系（J774 B10），通过延时摄影技术研究了小鼠巨噬细胞在小鼠血清中与白色念珠菌孢子的运动关系。巯基乙酸盐诱导的巨噬细胞和细胞系对孢子反应良好，但很少有常驻巨噬细胞表现出任何反应。巨噬细胞对孢子表现出趋化反应，呈直线向孢子移动。经常铺展在玻璃上的巯基乙酸盐诱导的巨噬细胞，也能通过向化性对20 - 30微米外的孢子做出反应，即伸出一个大的透明面纱来吞噬孢子，而细胞体无需事先移动。然后将被吞噬的孢子拉到富含细胞器的细胞中心。在这个群体中，铺展形态和运动行为之间不存在不相容性。相比之下，J774 B10不会铺展，呈圆形形态移动，朝着梯度源伸出非常小的前部透明面纱。巨噬细胞的移动速度比外周血中性粒细胞和单核细胞慢。巯基乙酸盐诱导的巨噬细胞和细胞系巨噬细胞的平均速度为每分钟3 - 4微米。少数有运动能力的常驻腹腔巨噬细胞移动得更慢，即每分钟约2微米。
