Tirosh R, Oplatka A
J Biochem. 1982 Apr;91(4):1435-40. doi: 10.1093/oxfordjournals.jbchem.a133832.
Solutions containing heavy meromyosin, actin, native tropomyosin, and Mg-ATP exhibited streaming in horizontally placed glass microcapillaries. Up-hill streaming could also be observed when the capillaries were at an inclined position; this served for the clear distinction between active and passive streaming provided surface tension effects were eliminated. The presence of native tropomyosin and actin-activation of the ATPase activity of HMM were essential for the reconstitution of active streaming. The significance of the results for cytoplasmic streaming and muscle contraction is discussed.
含有重酶解肌球蛋白、肌动蛋白、天然原肌球蛋白和Mg-ATP的溶液在水平放置的玻璃微毛细管中呈现出流动现象。当毛细管处于倾斜位置时，也能观察到向上流动；如果消除表面张力的影响，这有助于清晰区分主动流动和被动流动。天然原肌球蛋白的存在以及肌动蛋白对重酶解肌球蛋白ATP酶活性的激活对于重建主动流动至关重要。文中讨论了这些结果对细胞质流动和肌肉收缩的意义。
