Rannels D E, Wartell S A, Watkins C A
Life Sci. 1982 May 17;30(20):1679-90. doi: 10.1016/0024-3205(82)90300-9.
Attempts to quantitate metabolism in the lung and other tissues using radioactive precursors may be subject to significant errors arising from inappropriate assumptions regarding precursor metabolism, compartmentation and specific radioactivity. This article reviews the type and magnitude of error which may complicate such measurements by presenting specific data from experiments using radioactive amino acids to estimate the rate of protein synthesis. The applicability of these observations to other metabolic systems is discussed briefly in order to develop a more general awareness of the errors which may result from incomplete validation of experimental measurements using radioisotopes.
使用放射性前体对肺和其他组织中的代谢进行定量分析时，由于对前体代谢、区室化和比放射性的假设不当，可能会产生重大误差。本文通过展示使用放射性氨基酸估计蛋白质合成速率的实验中的具体数据，回顾了可能使此类测量复杂化的误差类型和程度。为了更普遍地认识到使用放射性同位素进行实验测量时，因验证不充分可能导致的误差，本文还简要讨论了这些观察结果对其他代谢系统的适用性。
