孵育和灌注肌肉中蛋白质合成与降解的比较。
Comparison of protein synthesis and degradation in incubated and perfused muscle.
作者信息
Clark A S, Mitch W E
出版信息
Biochem J. 1983 Jun 15;212(3):649-53. doi: 10.1042/bj2120649.
Abstract
Rates of muscle protein synthesis and degradation measured in the perfused hindquarter were compared with those in incubated epitrochlearis muscles. With fed or starved mature rats, results without insulin treatment were identical. With insulin treatment, protein synthesis in perfused hindquarters was greater, though protein degradation was the same. Thus rates of muscle protein degradation estimated by these two methods in vitro correspond closely.
摘要
将灌注后肢中测得的肌肉蛋白质合成与降解速率，与孵育的肱三头肌中的速率进行比较。对于喂食或饥饿的成年大鼠，未进行胰岛素处理时结果相同。进行胰岛素处理时，灌注后肢中的蛋白质合成较多，尽管蛋白质降解情况相同。因此，这两种体外方法估算的肌肉蛋白质降解速率密切相关。
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