Graybill J R, Straus D C, Nealon T J, Hague M, Paque R E
Mycopathologia. 1982 Apr 23;78(1):31-9. doi: 10.1007/BF00436579.
Cryptococcus neoformans cell and culture supernatant extracts were fractionated by ion exchange and gel filtration column chromatography. Various fractions were used to immunize mice, and to assess the release of migration inhibition factor, delayed type hypersensitivity, and protective immunity after challenge with C. neoformans. Results suggest that the C. neoformans fractions, which protect mice, contain a high molecular weight, predominantly carbohydrate antigen that can be distinguished from the capsular polysaccharide.
新型隐球菌细胞和培养上清液提取物通过离子交换和凝胶过滤柱色谱进行分级分离。使用各种级分免疫小鼠，并评估迁移抑制因子的释放、迟发型超敏反应以及用新型隐球菌攻击后的保护性免疫。结果表明，保护小鼠的新型隐球菌级分含有一种高分子量、主要为碳水化合物的抗原，该抗原可与荚膜多糖区分开来。
