Rosendahl M S, Omann G M, Leonard N J
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3480-4. doi: 10.1073/pnas.79.11.3480.
We describe here the synthesis and chemical properties of linear(lin)-benzoadenosylcobalamin, a coenzyme B12 analogue that has a laterally extended nucleoside in the upper axial position. It is an effective competitive inhibitor of ribonucleotide reductase from Lactobacillus leichmannii. lin-Benzoadenosylcobalamin is nonfluorescent in solution but, on homolytic (light) or heterolytic (acid, cyanide) cleavage of the carbon-cobalt bond, forms fluorescent products. In addition, fluorescence is detectable on binding of the coenzyme analogue to ribonucleotide reductase, and the observed fluorescence polarization of the lin-benzoadenosyl moiety indicates that it is bound loosely to the enzyme when the coenzyme is partially dissociated.
我们在此描述线性（lin）-苯并腺苷钴胺素的合成及其化学性质，它是一种辅酶B12类似物，在上轴向位置具有横向延伸的核苷。它是莱氏乳杆菌核糖核苷酸还原酶的有效竞争性抑制剂。线性-苯并腺苷钴胺素在溶液中无荧光，但在碳-钴键发生均裂（光照）或异裂（酸、氰化物）时会形成荧光产物。此外，在辅酶类似物与核糖核苷酸还原酶结合时可检测到荧光，并且观察到的线性-苯并腺苷部分的荧光偏振表明，当辅酶部分解离时，它与酶的结合较为松散。
