de Jong M F, Wellenberg G, Borst G H, Pomper W
Tijdschr Diergeneeskd. 1982 Jun 1;107(11):416-21.
During eleven visits to nine pig-breeding farms and multiplying herds, 190 piglets were sampled by three different methods. The nares were separately sampled and both nares were sampled with a single swab. Three media were used to discover which method and which medium produced the highest isolation-score for Bordetella bronchiseptica (BB). The CDI-Mac Conkey (McC-CDI) plate was used as a reference medium and was compared with the Gezondheidsdienst Mac Conkey (McC-GD) and the new Bordetella plate (Bord.pl). Of the 570 samples collected 303 were positive for Bordetella bronchiseptica. When the McC-CDI BB was used, the score was 286, when the McC-GD was used, it was 264 and when the Bord.pl. was used 277, and BB was present on each of the three plates in 243 cases. When both nares of each piglet were separately sampled, 94 per cent, 87 per cent and 85 per cent of the BB positive-piglets were found to be positive with respectively McC-CDI, McC-GD and the Bord.pl. Sampling each nare with one swab, produced an average BB score of approximately 80 per cent and both nares with a single swab approximately 85 per cent of the BB-positive pigs. Statistical testing showed the McC-CDI to be significantly (P = 0,05) superior to the Bord.pl.
在对9个养猪场和繁殖猪群进行的11次走访中，采用三种不同方法对190头仔猪进行了采样。分别对仔猪的两个鼻孔进行采样，并用一根拭子对两个鼻孔同时采样。使用三种培养基来确定哪种方法和哪种培养基对支气管败血波氏杆菌（BB）产生的分离得分最高。将CDI-麦康凯（McC-CDI）平板用作参考培养基，并与卫生服务麦康凯（McC-GD）和新型波氏杆菌平板（Bord.pl）进行比较。在采集的570个样本中，有303个样本支气管败血波氏杆菌呈阳性。使用McC-CDI BB时，得分是286；使用McC-GD时，得分是264；使用Bord.pl时，得分是277，三种平板中均有243例检测到BB。当对每头仔猪的两个鼻孔分别采样时，分别使用McC-CDI、McC-GD和Bord.pl检测出的BB阳性仔猪比例分别为94%、87%和85%。用一根拭子对每个鼻孔采样，BB的平均得分约为80%；用一根拭子对两个鼻孔同时采样，BB阳性猪的比例约为85%。统计检验表明，McC-CDI显著优于Bord.pl（P = 0.05）。
