Smith I M, Baskerville A J
Res Vet Sci. 1979 Sep;27(2):187-92.
A medium was developed that in most instances allowed the isolation from pigs of Bordetella (Alcaligenes) bronchiseptica in pure, or virtually pure, culture from such sites as the nasal cavity, which contains many other bacteria. If not suppressed, some of the latter can inhibit, sometimes completely, the growth of the bordetellae on artificial media. Besides being markedly selective, the new medium is simple to prepare, reasonably cheap and practically noninhibitory to B bronchiseptica. This organism produces a distinctive colony that is easily differentiated on morphological grounds from the few other bacteria (such as Alcaligenes faecalis and Pseudomonas spp) which also grow on this medium. Parallel examination of specimens from the nasal cavities of 219 pigs in the field in southern England confirmed that this medium was better than hitherto recommended for the detection of B bronchiseptica. This was particularly true when the organism occurred in small numbers only and/or when bacteria little affected by the inhibitors in the previously recommended media were present in the nasal flora.
已研发出一种培养基，在大多数情况下，能让猪的支气管败血博德特氏菌（产碱杆菌属）从鼻腔等部位分离出来，形成纯培养物或近乎纯的培养物，而鼻腔中含有许多其他细菌。如果不加以抑制，其中一些细菌有时会完全抑制支气管败血博德特氏菌在人工培养基上的生长。这种新培养基除了具有显著的选择性外，制备简单，成本合理，对支气管败血博德特氏菌几乎没有抑制作用。这种菌会产生一种独特的菌落，基于形态学很容易与也能在这种培养基上生长的其他少数细菌（如粪产碱杆菌和假单胞菌属）区分开来。对英格兰南部219头猪鼻腔样本的平行检测证实，这种培养基比迄今推荐用于检测支气管败血博德特氏菌的培养基更好。当该菌数量很少和/或鼻腔菌群中存在对先前推荐培养基中的抑制剂影响较小的细菌时，情况尤其如此。
